Fecha/hora: 01/06/2024 19:15:08

Biblioteca(s): | INIA Las Brujas.
Fecha corriente: | 17/04/2024
Fecha de la última actualización: | 17/04/2024
Autoria: | AREVALO, A.P.; BASIKA, T.; ANCHETA, S.; PERELMUTER, K.; RICCIARDI, A.; BOLLATI-FOGOLÍN, M.; CRISPO, M.
Afiliación: | ANA PAULA AREVALO, Institut Pasteur de Montevideo, Laboratory Animals Biotechnology Unit, Montevideo, Uruguay; TATIANA BASIKA, Unidad Mixta entre Instituto Pasteur de Montevideo e INIA (UMPI), Montevideo, Uruguay; Institut Pasteur de Montevideo, Laboratory Animals Biotechnology Unit, Montevideo, Uruguay; Institut Pasteur de Montevideo, Cell Biology Unit, Montevideo, Uruguay; SERGIO ANCHETA, Institut Pasteur de Montevideo, Laboratory Animals Biotechnology Unit, Montevideo, Uruguay; KAREN PERELMUTER, Institut Pasteur de Montevideo, Cell Biology Unit, Montevideo, Uruguay; ALEJANDRO RICCIARDI; MARIELA BOLLATI-FOGOLÍN, Institut Pasteur de Montevideo, Cell Biology Unit, Montevideo, Uruguay; MARTINA CRISPO, Institut Pasteur de Montevideo, Laboratory Animals Biotechnology Unit, Montevideo, Uruguay.
Título: | 3R's applied to in vivo biological activity of recombinant human erythropoietin assay.
Complemento del título: | Technical Article.
Año de publicación: | 2023
Fuente/Imprenta: | Biological Models Research and Technology Journal. 2023, Volume 3, Issue 1, e00012023. http://dx.doi.org/10.4322/2675-9225.00012023 -- OPEN ACCESS.
ISSN: | 2675-9225
DOI: | 10.4322/2675-9225.00012023
Idioma: | Inglés

Notas:

Article history: Submitted date 9 February 2023, Accepted date 19 April 2023. -- Correspondence: Crispo, M.; Institut Pasteur de Montevideo, Laboratory Animals Biotechnology Unit, Montevideo, Uruguay; email:crispo@pasteur.edu.uy -- FUNDING: This work was supported by FOCEM (MERCOSUR Structural Convergence Fund), COF 03/11 and ANII (EQL_2013_X_1_2). -- Document type: Article Bronze Open Access. -- 
Publisher: Brazilian Society for Laboratory Animal Science.

Contenido: | ABSTRACT.- An enhanced protocol for the evaluation of in vivo biological activity of recombinant human erythropoietin (rhEPO) assay in mice is described, following European Pharmacopoeia (Ph. Eur.) version 10.0 guideline and optimized by applying the principles of Replacement, Reduction and Refinement (3Rs) in animal experimentation. In Laboratory Animal Science, one of the main principles is to optimize the experimental protocols in order to achieve the best results with the lowest number of animals and refine the procedures to avoid unnecessary suffering. The main objective of this protocol is to comply with international guidelines for rhEPO evaluation, applying refinement on procedures and reduction in animal use. Some of the features included in this protocol are the increase in number of rhEPO batches tested simultaneously against an international standard, leading to a substantial reduction in the number of animal's used, and refinement on animal handling techniques for subcutaneous drug administration and blood withdrawal. The implemented improvements were validated by reticulocyte estimation to ensure compliance with international criteria established for this trial and institutional quality management system. © 2023, Brazilian Society for Laboratory Animal Science. All rights reserved.
Palabras-Clave: | 3R’s; Biological activity; Erythropoietin; European Pharmacopeia; Mice; UNIDAD MIXTA PASTEUR + INIA.
Categoría del tema: | A50 Investigación agraria
URL: | https://bmrtsbcaljournal.com/article/10.4322/2675-9225.00012023/pdf/bmrt-3-1-e00012023.pdf
Registro original: | INIA Las Brujas (LB)
Ejemplares: | LB - (103942-1) - PXI - AP - DD - Biol. M. Res. & Tech./2023 -  -  - DIS